PubMed ID:
19956101
Public Release Type:
Journal
Publication Year: 2009
Affiliation: Division of Public Health Sciences, Department of Biostatistics, Wake Forest University Health Sciences, Winston-Salem, NC 27157, USA. wmbrown@wfubmc.edu
DOI:
https://doi.org/10.1038/gene.2009.85
Authors:
Brown WM,
de Bakker PI,
Hilner JE,
Irenze K,
Lohman K,
Lu L,
Mirel DB,
Perdue LH,
Pierce JJ,
Type I Diabetes Genetics Consortium,
Ziaugra L
Studies:
Type 1 Diabetes Genetics Consortium
The Type I Diabetes Genetics Consortium (T1DGC) Rapid Response Workshop was established to evaluate published candidate gene associations in a large collection of affected sib-pair (ASP) families. We report on our quality control (QC) and preliminary family-based association analyses. A random sample of blind duplicates was analyzed for QC. Quality checks, including examination of plate-panel yield, marker yield, Hardy-Weinberg equilibrium, mismatch error rate, Mendelian error rate, and allele distribution across plates, were performed. Genotypes from 2324 families within nine cohorts were obtained from a panel of 21 candidate genes, including 384 single-nucleotide polymorphisms on two genotyping platforms performed at the Broad Institute Center for Genotyping and Analysis (Cambridge, MA, USA). The T1DGC Rapid Response project, following rigorous QC procedures, resulted in a 2297 family, 9688 genotyped individual database on a single-candidate gene panel. The available data include 9005 individuals with genotype data from both platforms and 683 individuals genotyped (276 in Illumina; 407 in Sequenom) on only one platform.